英语翻译1、Given that highly lipophilic LFP were the target molecules of the analysis,our approach concerning the finding the optimal separation conditions was to use more non-polar mobile phase than it is the case with the majority reverse phas

英语翻译1、Given that highly lipophilic LFP were the target molecules of the analysis,our approach concerning the finding the optimal separation conditions was to use more non-polar mobile phase than it is the case with the majority reverse phas
英语翻译
1、Given that highly lipophilic LFP were the target molecules of the analysis,our approach concerning the finding the optimal separation conditions was to use more non-polar mobile phase than it is the case with the majority reverse phase HPLC separations.
previous experiments in our laboratory with LFP showed an optimal isocratic elution with an acetonitrile-methanol-water mixture having different ratios of these solvents,depending on a tissue from which analyzed LFP originated.
This was adopted for the separation of LFP mixtures from human erythrocytes.
The optimal isocratic separation was there¬fore with the mobile phase consisting of 60% organic solvents and 40% water.
Higher percentage of organic solvents gave an incomplete separation with some peaks co-eluting,while higher percentage of water in the mobile phase resulted in peak broadening and prolongation of the analysis.
As for the injection volume,the optimum for a quali¬tative separation of LFP from the erythrocytes proved to be 20 μL.
With an injection volume of 30μL,the peaks in the chromatogram were poorly separated,given that a high concentration of analytes can result in broad peaks and co-elution,while 10 μL were in the other hand too low.
The excitation/emission wavelengths of 335/360 nm set on the detector were in accordance with a maximum differ¬ence in fluorescence intensity between the two groups of samples.
The flow rate of 0.4 mL min-1 was accepted for this separation after a few flow rates between 0.2 and 0.6 mL min-1 had been tried out.
Higher flow rates tended to produce high backpressure.
2、Method Development for a Qualitative Analysis of LFP from Beef Heart Mitochondria
The chromatographic separation was carried out with an injection of chloroform LFP extracts into the system with¬out prior solvent evaporation,for chloroform by no means worsened the separation.
When choosing the optimal mobile phase,we started with different mixtures of aceto- nitrile,methanol and water that proved to be good choices as running phases in our previous experiments.
However,LFP mixtures from mitochondria were not resolved well when using various combinations of these solvents.
After that,we did experiments with several different mobile phases composed of either methanol and water or aceto- nitrile and water,where the percentage of the non-polar solvent ranged from 30 to 80%.
The mobile phases con¬sisting of methanol and water were not efficient enough to clearly separate all fractions.
Using a mobile phase with acetonitrile as a non-polar solvent resulted in a better sep¬aration of different peaks,where the actual volume fraction of acetonitrile depended on the analyzed samples.
3、Comparing the chromato¬grams obtained with different volume fractions of acetonitrile used,it was found that mobile phase made of 60% acetonitrile,gave the best separation of individual peaks.

英语翻译1、Given that highly lipophilic LFP were the target molecules of the analysis,our approach concerning the finding the optimal separation conditions was to use more non-polar mobile phase than it is the case with the majority reverse phas
1,鉴于LFP的高度亲脂性分析的目标分子,我们的方法关于寻找最佳的分离条件是使用非极性流动相比,它是多数反相高效液相色谱法分离的情况下.
在我们的实验室与LFP以前的实验表明,最优等度洗脱,乙腈 - 甲醇 - 水混合物,这些溶剂的不同比例,取决于组织从分析LFP的起源.
这是通过从人红细胞LFP的混合物的分离.
最优等度分离有前用60％的有机溶剂组成的流动相和40％的水.
有机溶剂的比例更高了一些山峰共同洗脱不完全分离,而水在流动相的比例较高,在扩大高峰期和延长的分析结果.
至于注射量,红细胞的quali/LFP的tative的分离的最佳证明是：20μL.
注射量为30μL,色谱峰分离较差,由于高浓度的分析物可能会导致广泛的山峰和合作洗脱,10微升,而在另一方面,过低.
探测器上设置的335/360 nm的激发/发射波长是按照最大的不同¬ENCE在两组样品之间荧光强度.
流量的0.4毫升min-1的被接受后,0.2和0.6之间的数流率毫升min-1的已尝试了这种分离.
较高的流速往往会产生高背压.
2,从牛心线粒体的LFP的定性分析方法开发
进行色谱分离,与氯仿LFP的提取物注射到¬出事先溶剂蒸发系统,绝不氯仿恶化的分离.
选择最佳的流动相时,我们开始与乙酰腈,甲醇和水被证明是很好的选择,在我们以前的实验运行阶段的不同混合物.
然而,从线粒体LFP的混合物没有得到解决时,使用这些溶剂的各种组合.
之后,我们做实验,与几个不同的流动相组成,甲醇和水或乙酰腈和水,在非极性溶剂的比例从30％至80％不等.
流动相甲醇和水的浓度¬sisting效率不高,不够明确分开的所有分数.
作为一个非极性溶剂乙腈流动相使用导致一个更好的九月¬aration不同的山峰,其中乙腈的实际体积分数取决于所分析的样品.
3,比较获得色谱用乙腈不同体积分数¬克,结果发现,60％乙腈的流动相,给个别峰的最佳分离.